双苄基异喹啉生物碱在小鼠皮肤两阶段致癌过程中可抑制12-O-十四烷酰佛波醇-13-乙酸酯的促癌作用。

Bisbenzylisoquinoline alkaloids inhibit tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin.

作者信息

Yasukawa K, Akasu M, Takeuchi M, Takido M

机构信息

College of Pharmacy, Nihon University, Chiba, Japan.

出版信息

Oncology. 1993 Mar-Apr;50(2):137-40. doi: 10.1159/000227165.

DOI:10.1159/000227165

PMID:8451036

Abstract

Bisbenzylisoquinoline alkaloids, cepharanthine, berbamine and isotetrandrine, were isolated from Stephania cepharantha Hayata. These compounds inhibit arachidonic acid-induced inflammation in mice. We have found that cepharanthine inhibits tumor promotion after topical application and oral administration in two-stage carcinogenesis in mouse skin. Furthermore, topical application of berbamine (2 mumol/mouse) and isotetrandrine (2 mumol/mouse) markedly suppressed the tumor-promoting effect of 12-O-tetradecanoylphorbol-13-acetate (1 microgram) in mouse skin initiated with 7,12-dimethylbenz[a]anthracene (50 micrograms), at a grade corresponding to that of cepharanthine.

摘要

双苄基异喹啉生物碱，汉防己甲素、小檗胺和异粉防己碱，是从中华千金藤中分离得到的。这些化合物可抑制花生四烯酸诱导的小鼠炎症。我们发现，在小鼠皮肤两阶段致癌过程中，局部应用和口服汉防己甲素均能抑制肿瘤促进作用。此外，局部应用小檗胺（2 μmol/小鼠）和异粉防己碱（2 μmol/小鼠）可显著抑制12-O-十四酰佛波醇-13-乙酸酯（1 μg）对用7,12-二甲基苯并[a]蒽（50 μg）启动的小鼠皮肤的促肿瘤作用，其抑制程度与汉防己甲素相当。