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Partial purification and characterization of a translational inhibitor from Friend leukemia cells.

作者信息

Pinphanichakarn P, Kramer G, Hardesty B

出版信息

J Biol Chem. 1977 Mar 25;252(6):2106-12.

PMID:845164
Abstract

A translational inhibitor has been isolated from unstimulated Friend leukemia cells. The inhibitor is a heat-labile, sulfhydryl reagent-insensitive protein with a molecular weight of approximately 214,000. It inhibits protein synthesis at a step of peptide chain initiation by preventing initiation factor-dependent binding of methionyl-tRNAf to 40 S ribosomal subunits. However, it does not interfere with the formation of the ribosome-independent ternary complex between the initiation factor IF-E2, methionyl-tRNAf, and GTP. The inhibitor preparation contains protein kinase activity which phosphorylates the smallest subunit of IF-E2. There appears to be a functional similarity between this inhibitor from Friend leukemia cells and the hemin-controlled repressor from reticulocytes.

摘要

相似文献

1
Partial purification and characterization of a translational inhibitor from Friend leukemia cells.
J Biol Chem. 1977 Mar 25;252(6):2106-12.
2
Functional relationships between a reticulocyte polypeptide-chain-initiation factor (IF-MP) and the translational inhibitor involved in regulation of protein synthesis by haemin.网织红细胞多肽链起始因子（IF-MP）与参与血红素调节蛋白质合成的翻译抑制剂之间的功能关系。
Eur J Biochem. 1976 Jul 1;66(2):413-22. doi: 10.1111/j.1432-1033.1976.tb10531.x.
3
Partial purification and characterization of a 90,000-dalton peptide involved in activation of the eIF-2 alpha protein kinase of the hemin-controlled translational repressor.参与血红素控制的翻译阻遏物的eIF-2α蛋白激酶激活的一种90,000道尔顿肽的部分纯化及特性鉴定
Biochemistry. 1980 Feb 19;19(4):798-804. doi: 10.1021/bi00545a028.
4
The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。
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5
Regulation of protein synthesis in rabbit reticulocyte lysates: additional initiation factor required for formation of ternary complex (eIF-2.GTP.Met-tRNAf) and demonstration of inhibitory effect of heme-regulated protein kinase.兔网织红细胞裂解物中蛋白质合成的调控：三元复合物（eIF-2·GTP·Met-tRNAf）形成所需的额外起始因子以及血红素调节蛋白激酶抑制作用的证明。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1079-83. doi: 10.1073/pnas.76.3.1079.
6
Preparation and characterization of eukaryotic initiation factor EIF-3. Formation of binary (EIF-3-Met-tRNAf) and ternary (EIF-3-Met-tRNAf-GTP) complexes.真核起始因子EIF-3的制备与表征。二元复合物（EIF-3-甲硫氨酰-tRNAf）和三元复合物（EIF-3-甲硫氨酰-tRNAf-GTP）的形成。
J Biol Chem. 1976 Apr 10;251(7):1926-35.
7
Regulation of protein synthesis by hemin. Evidence that the hemin-controlled translational repressor inhibits the rate of formation of 40S.Met-tRNAf complexes directly.血红素对蛋白质合成的调控。有证据表明，血红素控制的翻译阻遏物直接抑制40S·Met-tRNAf复合物的形成速率。
J Biol Chem. 1979 Apr 10;254(7):2378-83.
8
Partial reaction of peptide initiation inhibited by phosphorylation of either initiation factor eIF-2 or 40S ribosomal proteins.起始因子eIF-2或40S核糖体蛋白的磷酸化抑制肽起始的部分反应。
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1445-9. doi: 10.1073/pnas.74.4.1445.
9
Stimulation of protein synthesis and Met-tRNAf binding by phosphorylated sugars: studies on their mechanism of action.磷酸化糖对蛋白质合成和甲硫氨酸-tRNAf结合的刺激作用：关于其作用机制的研究
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10
Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation.网织红细胞无细胞系统引发多肽链。翻译的不同抑制剂研究。
Eur J Biochem. 1976 Sep 15;68(2):355-64. doi: 10.1111/j.1432-1033.1976.tb10822.x.

引用本文的文献

1
Hemin-independent control of globin synthesis in Friend erythroleukemia cells induced to differentiate.在诱导分化的弗氏红白血病细胞中，珠蛋白合成的血红素非依赖性调控。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1839-43. doi: 10.1073/pnas.79.6.1839.
2
Activation of the heme-stabilized translational inhibitor of reticulocyte lysates by calcium ions and phospholipid.钙离子和磷脂对网织红细胞裂解液中血红素稳定化翻译抑制剂的激活作用。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6843-7. doi: 10.1073/pnas.80.22.6843.
3
Alkaline phosphatase and protein kinase(s) activities in free cytoplasmic mRNPs from human term placenta.
人足月胎盘游离细胞质信使核糖核蛋白中的碱性磷酸酶和蛋白激酶活性
Mol Biol Rep. 1986;11(1):29-35. doi: 10.1007/BF00417592.
4
Presence of haemin-controlled eIF-2 alpha kinases in both undifferentiated and differentiating mouse erythroleukaemia cells.未分化和正在分化的小鼠红白血病细胞中均存在血红素控制的真核起始因子2α激酶。
Biochem J. 1989 Sep 1;262(2):569-74. doi: 10.1042/bj2620569.
5
All factors required for protein synthesis are retained on heparin bound to Sepharose.蛋白质合成所需的所有因子都保留在与琼脂糖结合的肝素上。
Biochem J. 1978 Apr 15;172(1):1-7. doi: 10.1042/bj1720001.
6
Translational control by protein kinase in Artemia salina and wheat germ.卤虫和小麦胚芽中蛋白激酶的翻译控制
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4356-9. doi: 10.1073/pnas.74.10.4356.
7
mRNP proteins, initiation factors and phosphorylation.
Mol Biol Rep. 1979 May 31;5(1-2):91-7. doi: 10.1007/BF00777494.