Sierra J M, de Haro C, Datta A, Ochoa S
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4356-9. doi: 10.1073/pnas.74.10.4356.
The catalytic subunit of cyclic 3':5'-AMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) inhibits translation in Artemia salina and wheat germ extracts. It acts, as in reticulocyte lysates [Datta, A., de Haro, C., Sierra, J. M. & Ochoa, S. (1977) Proc. Natl. Acad. Sci. USA 74, 1463-1467] by catalyzing the conversion of a proinhibitor to an inhibitor of polypeptide chain initiation. Addition of ATP and either cyclic AMP or catalytic subunit promotes the proinhibitor-inhibitor conversion in crude proinhibitor preparations from A. salina embryos. The effect of cyclic AMP is due to stimulation of cyclic AMP-dependent protein kinase, present in such preparations, and is inhibited by hemin. In similar preparations from wheat germ, addition of ATP and catalytic subunit promoted proinhibitor-inhibitor conversion, but addition of ATP and cyclic AMP has little or no effect. As assayed with histone as substrate, wheat germ preparations exhibit a protein kinase activity that is not stimulated by the addition of cyclic AMP or cyclic GMP. Our results suggest that a translational control system, similar to that existing in rabbit reticulocytes and other mammalian cells, is present in organisms evolutionarily far removed from mammals.
环化3':5'-AMP依赖性蛋白激酶（ATP：蛋白磷酸转移酶，EC 2.7.1.37）的催化亚基可抑制卤虫和小麦胚芽提取物中的翻译过程。其作用方式与网织红细胞裂解物中的情况相同[达塔，A.，德阿罗，C.，西拉，J.M.和奥乔亚，S.（1977年）《美国国家科学院院刊》74卷，第1463 - 1467页]，即通过催化一种前抑制剂转化为多肽链起始的抑制剂来发挥作用。添加ATP以及环化AMP或催化亚基可促进来自卤虫胚胎的粗制前抑制剂制剂中的前抑制剂 - 抑制剂转化。环化AMP的作用是由于刺激了此类制剂中存在的环化AMP依赖性蛋白激酶，并且该作用被血红素抑制。在来自小麦胚芽的类似制剂中，添加ATP和催化亚基可促进前抑制剂 - 抑制剂转化，但添加ATP和环化AMP几乎没有作用或根本没有作用。以组蛋白作为底物进行测定时，小麦胚芽制剂表现出一种蛋白激酶活性，添加环化AMP或环化GMP并不能刺激该活性。我们的结果表明，一种类似于存在于兔网织红细胞和其他哺乳动物细胞中的翻译控制系统，存在于与哺乳动物在进化上相距甚远的生物体中。
