通过苯异硫氰酸酯进行柱前衍生化分析神经毒素β-ODAP及其α-异构体。
Analysis of the neurotoxin beta-ODAP and its alpha-isomer by precolumn derivatization with phenylisothiocyanate.
作者信息
Khan J K, Kebede N, Kuo Y H, Lambein F, De Bruyn A
机构信息
Laboratory of Physiological Chemistry, Faculty of Medicine, University of Ghent, Belgium.
出版信息
Anal Biochem. 1993 Feb 1;208(2):237-40. doi: 10.1006/abio.1993.1038.
A method is presented for determining 3-N-oxalyl-2,3-diaminopropanoic acid (beta-ODAP), the most potent neurotoxic substance of the seeds and seedlings of Lathyrus sativus, and its much less toxic 2-isomer (alpha-ODAP). The separation of the two forms is achieved after derivatization with phenylisothiocyanate employing a HPLC system. This method was used to monitor the isomerization of beta-ODAP to alpha-ODAP at different time intervals and to quantify the toxin level in seed extracts.
本文介绍了一种测定3-N-草酰基-2,3-二氨基丙酸（β-ODAP）的方法，β-ODAP是草豌豆种子和幼苗中最具神经毒性的物质，以及毒性小得多的2-异构体（α-ODAP）。采用高效液相色谱系统，用异硫氰酸苯酯衍生化后，实现了两种形式的分离。该方法用于监测不同时间间隔下β-ODAP向α-ODAP的异构化，并对种子提取物中的毒素水平进行定量。
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