Muzykantov V R, Smirnov M D, Zaltzman A B, Samokhin G P
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Anal Biochem. 1993 Feb 1;208(2):338-42. doi: 10.1006/abio.1993.1057.
It was shown previously that avidin attachment to biotinylated erythrocytes induces their lysis by homologous complement via the alternative pathway. This phenomenon hinders the use of avidin-coated immunoerythrocytes as carriers for drug targeting. In the present work we demonstrated that attachment of avidin to erythrocytes via the cross-linking agent tannin does not induce their lysis by complement. Tannization allows attachment of about 5 x 10(5) molecules of avidin per erythrocyte, which is comparable to the value obtained after treatment with biotin esters. In contrast to biotinylated avidin-coated erythrocytes, tannized avidin-coated erythrocytes were not lysed by complement. Tannization itself does not reduce the erythrocyte sensitivity to lysis by complement in the presence of activators of the complement (hemolytic antibody or activators of the alternative pathway). Therefore, the avidin-induced lysis by complement depends on the mode of avidin attachment to erythrocyte. Complement-resistant tannized erythrocytes coated with avidin bind biotinylated immunoglobulins (to 7 x 10(4) molecules per cell), suggesting that tannization might be used for the preparation of complement-resistant immunoerythrocytes.
先前已表明，抗生物素蛋白附着于生物素化红细胞会通过替代途径诱导其被同源补体溶解。这种现象阻碍了将抗生物素蛋白包被的免疫红细胞用作药物靶向载体。在本研究中，我们证明通过交联剂单宁酸使抗生物素蛋白附着于红细胞不会诱导其被补体溶解。单宁化可使每个红细胞附着约5×10⁵个抗生物素蛋白分子，这与用生物素酯处理后获得的值相当。与生物素化的抗生物素蛋白包被的红细胞不同，单宁化的抗生物素蛋白包被的红细胞不会被补体溶解。单宁化本身在存在补体激活剂（溶血抗体或替代途径激活剂）的情况下不会降低红细胞对补体溶解的敏感性。因此，抗生物素蛋白诱导的补体溶解取决于抗生物素蛋白附着于红细胞的方式。包被有抗生物素蛋白的抗补体溶解的单宁化红细胞可结合生物素化免疫球蛋白（每个细胞可达7×10⁴个分子），这表明单宁化可用于制备抗补体溶解的免疫红细胞。
