Clark I M, Powell L K, Ramsey S, Hazleman B L, Cawston T E
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, United Kingdom.
Arthritis Rheum. 1993 Mar;36(3):372-9. doi: 10.1002/art.1780360313.
To compare the levels of collagenase, tissue inhibitor of metalloproteinases (TIMP), and collagenase-TIMP complex in synovial fluid (SF) from patients with rheumatoid arthritis (RA) and patients with osteoarthritis (OA). This study aims to clarify existing data from previously used enzyme or inhibitor activity assays performed following separation by gel filtration, by using a 1-step enzyme-linked immunosorbent assay (ELISA) for each component.
Total collagenase, free TIMP, and collagenase-TIMP complex were measured using a newly developed, specific double-antibody sandwich ELISA:
Levels of both collagenase and TIMP were significantly higher in RA patients (collagenase 1,560 +/- 150 ng/ml [mean +/- SEM], TIMP 1,610 +/- 130 ng/ml; n = 80) than OA patients (collagenase 420 +/- 90 ng/ml, TIMP 1,050 +/- 60 ng/ml; n = 80), with the difference being especially striking for collagenase. Sixteen RA fluids had detectable levels of collagenase-TIMP complex, compared with only 3 OA fluids.
The level of total collagenase in SF is greater in RA than OA, while levels of free TIMP show more overlap between the 2 diseases; this may simply reflect the increased inflammation seen in RA, or it may reflect a different disease mechanism.
比较类风湿关节炎（RA）患者和骨关节炎（OA）患者滑液（SF）中胶原酶、金属蛋白酶组织抑制剂（TIMP）及胶原酶 - TIMP复合物的水平。本研究旨在通过对每种成分使用一步法酶联免疫吸附测定（ELISA），阐明先前通过凝胶过滤分离后进行的酶或抑制剂活性测定的现有数据。
使用新开发的特异性双抗体夹心ELISA测定总胶原酶、游离TIMP和胶原酶 - TIMP复合物。
RA患者（胶原酶1,560±150 ng/ml[平均值±标准误]，TIMP 1,610±130 ng/ml；n = 80）的胶原酶和TIMP水平均显著高于OA患者（胶原酶420±90 ng/ml，TIMP 1,050±60 ng/ml；n = 80），胶原酶的差异尤为显著。16份RA滑液中可检测到胶原酶 - TIMP复合物水平，而OA滑液中只有3份可检测到。
RA患者滑液中总胶原酶水平高于OA患者，而游离TIMP水平在这两种疾病之间有更多重叠；这可能仅仅反映了RA中炎症的增加，或者可能反映了不同的疾病机制。
