Bodine A B, Davis R W, Janzen J J, Crawford F M
J Dairy Sci. 1977 Mar;60(3):450-2. doi: 10.3168/jds.s0022-0302(77)83886-1.
The cellulose chromatography method of the Association of Official Analytical Chemists for determination of Aflatoxin M1 was compared with a two-dimensional thin layer chromatography procedure. The minimum detectable Aflatoxin M1 by the Official procedure was approximately .01 mug/kg while the thin layer chromatography procedure has a claimed sensitivity of .004 mug/kg. Analyses of milk samples by thin layer chromatography agreed with results of the Official procedure in the range of .01 to .25 mug/liter. Each method consistently yielded recoveries greater than 87% and there was no significant difference between means or variances of each method. The thin layer chromatography method took less time than the Official method and showed a reproducible lower threshold sensitivity of .005 mug/liter.
将官方分析化学家协会用于测定黄曲霉毒素M1的纤维素色谱法与二维薄层色谱法进行了比较。官方方法可检测到的黄曲霉毒素M1的最低含量约为0.01微克/千克，而薄层色谱法宣称的灵敏度为0.004微克/千克。用薄层色谱法对牛奶样品进行分析，其结果与官方方法在0.01至0.25微克/升范围内的结果一致。每种方法的回收率始终大于87%，且每种方法的均值或方差之间无显著差异。薄层色谱法比官方方法耗时少，且显示出可重现的更低检测限灵敏度，为0.005微克/升。
