Christensen T, Kinn G
Medical Section, National Institute of Radiation Hygiene, Osterås, Norway.
Acta Paediatr. 1993 Jan;82(1):22-5. doi: 10.1111/j.1651-2227.1993.tb12508.x.
Cultured cells from one human and one murine cell line were incubated with bilirubin by different methods that allowed bilirubin to be bound to cells. The cells were irradiated with visible light of different wavelengths. Bilirubin bound to human serum albumin was also irradiated with light. After irradiation, bilirubin and its photoisomers were extracted and analyzed by HPLC. No photoisomers were found in samples of irradiated cells, while the types and amounts of photoisomers that were expected from the literature were found in samples of irradiated bilirubin/albumin mixtures. We conclude that the formation of therapeutically active photoisomers during phototherapy most probably does not take place in skin cells, but most likely in bilirubin bound to albumin in the vessels or in the interstitial space.
来自一种人类细胞系和一种鼠类细胞系的培养细胞，通过不同方法与胆红素一起孵育，这些方法可使胆红素与细胞结合。用不同波长的可见光照射这些细胞。与人类血清白蛋白结合的胆红素也进行光照。照射后，提取胆红素及其光异构体，并通过高效液相色谱法进行分析。在照射细胞的样本中未发现光异构体，而在照射胆红素/白蛋白混合物的样本中发现了文献中预期的光异构体类型和数量。我们得出结论，光疗期间治疗活性光异构体的形成很可能不在皮肤细胞中发生，而最有可能发生在血管或间质空间中与白蛋白结合的胆红素中。
