Onishi S, Itoh S, Yamakawa T, Isobe K, Manabe M, Toyota S, Imai T
Biochem J. 1985 Sep 15;230(3):561-7. doi: 10.1042/bj2300561.
It has been stated by McDonagh, Palma & Lightner [(1982) J. Am. Chem. Soc. 104, 6867-6871] that complexing of bilirubin with serum albumin has a marked species-dependent influence on bilirubin photoisomerization in vitro and in vivo. Therefore the kinetics for the quantitatively important reaction: (Formula: see text) of the photochemical interconversion between bilirubin and its photoisomers bound to human or rat serum albumin in aqueous solution, assayed by h.p.l.c., was used to elucidate the observed species-dependent difference. The relative rate constants for bilirubin bound to human serum albumin, except for k4, the rate of interconversion from (ZZ)-bilirubin into (EZ)-bilirubin, proved to be considerably larger than those for bilirubin bound to rat serum albumin. In accordance with these rate constants, the formation of photoisomers of bilirubin bound to human serum albumin, except for (EZ)-bilirubin, is very rapid and much greater than that for bilirubin bound to rat serum albumin.
麦克多纳、帕尔马和莱特纳[（1982年）《美国化学会志》104，6867 - 6871]指出，胆红素与血清白蛋白的络合在体外和体内对胆红素的光异构化具有显著的物种依赖性影响。因此，通过高效液相色谱法测定了水溶液中胆红素与其与人或大鼠血清白蛋白结合的光异构体之间光化学相互转化的重要定量反应（公式：见原文）的动力学，以阐明观察到的物种依赖性差异。除了从（ZZ） - 胆红素转化为（EZ） - 胆红素的速率k4外，与人血清白蛋白结合的胆红素的相对速率常数被证明比与大鼠血清白蛋白结合的胆红素的相对速率常数大得多。根据这些速率常数，除了（EZ） - 胆红素外，与人血清白蛋白结合的胆红素光异构体的形成非常迅速，且比与大鼠血清白蛋白结合的胆红素的形成要多得多。
