Böhm M, Wieland I, Totzeck B
Institut für Zellbiologie (Tumorforschung), Universität, Essen, Germany.
Cancer Genet Cytogenet. 1993 Feb;65(2):83-7. doi: 10.1016/0165-4608(93)90211-4.
Cancer is associated with homozygous deletions of specific DNA sequences that are in some instances too small to be detectable by cytogenetic methods or Southern blot analysis. Such small tumor-specific deletions can be detected, however, by the polymerase chain reaction (PCR) provided that tumor cells are meticulously and verifiably isolated from contaminating nontumor cells. Nontumor cells can give positive PCR results and thus obscure the detection of deletions. Using a method that allows accurate and verifiable excision of tumor cells for subsequent PCR analysis, homozygous deletions, one in a putative tumor suppressor gene on chromosome 8 and one in the p53 gene, were detected in two out of 20 human lung carcinomas investigated.
癌症与特定DNA序列的纯合缺失有关，这些缺失在某些情况下太小，无法通过细胞遗传学方法或Southern印迹分析检测到。然而，只要从污染的非肿瘤细胞中精心且可验证地分离出肿瘤细胞，就可以通过聚合酶链反应（PCR）检测到这种小的肿瘤特异性缺失。非肿瘤细胞可能会给出阳性PCR结果，从而掩盖缺失的检测。使用一种能够准确且可验证地切除肿瘤细胞以进行后续PCR分析的方法，在研究的20例人类肺癌中，有2例检测到纯合缺失，其中1例位于8号染色体上的一个假定肿瘤抑制基因中，另1例位于p53基因中。
