Böhm M, Wieland I, Schütze K, Rübben H
Urologische Klinik, Universitätsklinikum Essen, Germany.
Am J Pathol. 1997 Jul;151(1):63-7.
The analysis of tissue-specific genetic alterations depends on the selective procurement of homogeneous cell populations. Microbeam microdissection of membrane-mounted native tissue (MOMeNT) permits the rapid, selective, and low-contamination procurement of tumor or other cells from histological sections by non-thermic non-contact laser microdissection. Tissue sections are mounted on a specifically designed ultrathin transparent supporter membrane. Tissue together with the membrane are then dissected with an ultraviolet (337-nm) pulsed laser microbeam coupled into a robot-stage microscope. The ultraviolet laser causes dissection by cold photolysis due to the high photon density of the microbeam rather than by local heating. The track of the laser microbeam can be preselected freely on a video screen, and the size and form of the dissectates can thus be adapted to the histological features of the section with a delineation accuracy in the micron range. Polymerase chain reaction amplification of DNA from the dissectates is not impaired, and tumor-specific loss of heterozygosity of the APC gene as well as homozygous deletion of the MTS1 gene are demonstrated in bladder carcinomas. Taken together, microbeam MOMeNT is a novel technique that utilizes membrane-based microdissection by an ultraviolet laser microbeam, thus providing a flexible, easy-to-use high-performance tool for the molecular pathologist.
组织特异性基因改变的分析依赖于均一细胞群体的选择性获取。膜载天然组织微束显微切割技术（MOMeNT）可通过非热非接触激光显微切割，从组织学切片中快速、选择性且低污染地获取肿瘤细胞或其他细胞。组织切片被置于专门设计的超薄透明支持膜上。然后，将组织连同该膜一起用耦合到机器人载物台显微镜的紫外（337纳米）脉冲激光微束进行切割。由于微束的高光子密度，紫外激光通过冷光解作用而非局部加热来实现切割。激光微束的轨迹可在视频屏幕上自由预选，因此所切割物的大小和形状可根据切片的组织学特征进行调整，其轮廓精度可达微米范围。从所切割物中提取的DNA进行聚合酶链反应扩增未受影响，并且在膀胱癌中证实了APC基因的肿瘤特异性杂合性缺失以及MTS1基因的纯合缺失。综上所述，微束MOMeNT是一种利用紫外激光微束进行基于膜的显微切割的新技术，从而为分子病理学家提供了一种灵活、易用的高性能工具。
