用于从杂交细胞中分离人类染色体涂染探针的双Alu聚合酶链反应引物及条件

Dual Alu polymerase chain reaction primers and conditions for isolation of human chromosome painting probes from hybrid cells.

作者信息

Liu P, Siciliano J, Seong D, Craig J, Zhao Y, de Jong P J, Siciliano M J

机构信息

Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston 77030.

出版信息

Cancer Genet Cytogenet. 1993 Feb;65(2):93-9. doi: 10.1016/0165-4608(93)90213-6.

DOI:10.1016/0165-4608(93)90213-6

PMID:8453610

Abstract

A method for rapid and efficient production of chromosome- and chromosome-region specific probes for fluorescent in situ hybridization (FISH) detectable by simple fluorescent microscopy is described. The procedure is based on simultaneous use of two inter-Alu-polymerase chain reaction (PCR) primers for extraction of highly heterogeneous human DNA from interspecific somatic cell hybrids containing the chromosome regions of interest. Probes so produced do not hybridize to centromeric sequences and simultaneously band the target chromosomes, making them useful for unambiguous identification of chromosomal elements and breakpoints associated with cancer.

摘要

本文描述了一种用于快速高效生产用于荧光原位杂交（FISH）的染色体和染色体区域特异性探针的方法，该探针可通过简单的荧光显微镜检测。该方法基于同时使用两种Alu间聚合酶链反应（PCR）引物，从含有感兴趣染色体区域的种间体细胞杂种中提取高度异质的人类DNA。如此产生的探针不会与着丝粒序列杂交，同时使目标染色体显带，使其可用于明确识别与癌症相关的染色体元件和断点。