Antonacci R, Marzella R, Finelli P, Lonoce A, Forabosco A, Archidiacono N, Rocchi M
Istituto di Genetica, Università di Bari, Italy.
Cytogenet Cell Genet. 1995;68(1-2):25-32. doi: 10.1159/000133882.
DNA samples from about 100 human-hamster somatic cell hybrids, previously characterized by conventional banding techniques, were amplified with dual-Alu PCR. The products were then used as probes in FISH experiments on normal human metaphases for an accurate cytogenetic characterization of the human material retained in each hybrid. In addition to entire chromosomes, most hybrids were found to contain one or a few chromosome fragments, as a result of rearrangements that had occurred in vitro. Forty additional primary hybrids, in which conventional cytogenetic analysis failed to reveal any complete human chromosome, contained many human chromosome fragments. More than 300 chromosome fragments were scored and their precise chromosomal location recorded. We show data indicating that subchromosomal painting libraries generated from these hybrids can be favorably used in the fine characterization of chromosomal rearrangements encountered in clinical cytogenetics or in tumor cytogenetics, and in tracking chromosomal changes that occurred in primate evolution.
从大约100个人-仓鼠体细胞杂种中提取的DNA样本，此前已通过传统显带技术进行了表征，使用双Alu PCR进行扩增。然后将产物用作探针，在正常人中期的荧光原位杂交（FISH）实验中，对每个杂种中保留的人类材料进行准确的细胞遗传学表征。除了完整的染色体外，由于体外发生的重排，大多数杂种被发现含有一个或几个染色体片段。另外40个初级杂种，在传统细胞遗传学分析中未能发现任何完整的人类染色体，但含有许多人类染色体片段。对300多个染色体片段进行了评分，并记录了它们的确切染色体位置。我们展示的数据表明，从这些杂种中产生的亚染色体涂染文库可有效地用于临床细胞遗传学或肿瘤细胞遗传学中遇到的染色体重排的精细表征，以及追踪灵长类进化过程中发生的染色体变化。
