Bertram M J, Bérubé N G, Hang-Swanson X, Ran Q, Leung J K, Bryce S, Spurgers K, Bick R J, Baldini A, Ning Y, Clark L J, Parkinson E K, Barrett J C, Smith J R, Pereira-Smith O M
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030, USA.
Mol Cell Biol. 1999 Feb;19(2):1479-85. doi: 10.1128/MCB.19.2.1479.
Based on the dominance of cellular senescence over immortality, immortal human cell lines have been assigned to four complementation groups for indefinite division. Human chromosomes carrying senescence genes have been identified, including chromosome 4. We report the cloning and identification of a gene, mortality factor 4 (MORF 4), which induces a senescent-like phenotype in immortal cell lines assigned to complementation group B with concomitant changes in two markers for senescence. MORF 4 is a member of a novel family of genes with transcription factor-like motifs. We present here the sequences of the seven family members, their chromosomal locations, and a partial characterization of the three members that are expressed. Elucidation of the mechanism of action of these genes should enhance our understanding of growth regulation and cellular aging.
基于细胞衰老对永生的主导地位，永生人类细胞系已被分为四个互补组以进行无限分裂。已鉴定出携带衰老基因的人类染色体，包括4号染色体。我们报告了一个基因——死亡因子4（MORF 4）的克隆和鉴定，该基因在分配到互补组B的永生细胞系中诱导出类似衰老的表型，并伴随两个衰老标记的变化。MORF 4是一个具有转录因子样基序的新基因家族的成员。我们在此展示了七个家族成员的序列、它们的染色体定位以及三个表达成员的部分特征。对这些基因作用机制的阐明应能增进我们对生长调控和细胞衰老的理解。
