大鼠脑钠钙交换基因两种亚型的克隆及其在HeLa细胞中的功能表达。

Cloning of two isoforms of the rat brain Na(+)-Ca2+ exchanger gene and their functional expression in HeLa cells.

作者信息

Furman I, Cook O, Kasir J, Rahamimoff H

机构信息

Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

出版信息

FEBS Lett. 1993 Mar 15;319(1-2):105-9. doi: 10.1016/0014-5793(93)80046-w.

DOI:10.1016/0014-5793(93)80046-w

PMID:8454039

Abstract

Two functional isoforms of the rat brain Na(+)-Ca2+ exchanger were isolated from a lambda ZAP hippocampus cDNA library. The open reading frame of clone RBE-1 codes for a protein 935 amino acids long, and that of clone RBE-2 codes for a protein 958 amino acids long. Expression HeLa cells of Na+ gradient dependent Ca2+ transport activity was determined following transfection of the cells with either RBE-1 or RBE-2. Both clones expressed proteins that exchange Na+ with Ca2+ in an electrogenic manner and none of them exhibited a dependency of the antiport on K, since they transported Ca2+ in an Na+ gradient dependent manner in external choline chloride as well.

摘要

从λZAP海马cDNA文库中分离出大鼠脑钠钙交换体的两种功能亚型。克隆RBE - 1的开放阅读框编码一个935个氨基酸长的蛋白质，克隆RBE - 2的开放阅读框编码一个958个氨基酸长的蛋白质。在用RBE - 1或RBE - 2转染细胞后，测定了表达钠梯度依赖性钙转运活性的HeLa细胞。两个克隆都表达了以电生方式进行钠钙交换的蛋白质，并且它们都没有表现出反向转运对钾的依赖性，因为它们在外部氯化胆碱中也以钠梯度依赖性方式转运钙。