Schmidt M, Walker R B, Hoffman D R, McConnell T J
Department of Pathology, East Carolina University, School of Medicine, Greenville, NC 27834.
FEBS Lett. 1993 Mar 15;319(1-2):138-40. doi: 10.1016/0014-5793(93)80053-w.
For the first time the cDNA encoding a fire ant venom protein has been sequenced. Oligonucleotides were designed according to the amino acid sequence. The cDNA sequence was obtained by hybridizing these primers to mRNA and enhancement by the PCR technique. Comparison to the amino acid sequence of the venom protein shows a leader sequence 19 amino acids long.
编码火蚁毒液蛋白的cDNA首次被测序。根据氨基酸序列设计了寡核苷酸。通过使这些引物与mRNA杂交并利用PCR技术进行扩增来获得cDNA序列。与毒液蛋白的氨基酸序列比较显示出一个长度为19个氨基酸的前导序列。
