虹鳟卵巢胆固醇侧链裂解细胞色素P450（P450scc）。卵子发生过程中的cDNA克隆及mRNA表达
Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.
作者信息
Takahashi M, Tanaka M, Sakai N, Adachi S, Miller W L, Nagahama Y
机构信息
Department of Molecular Biomechanics, Graduate University for Advanced Studies, Okazaki, Japan.
出版信息
FEBS Lett. 1993 Mar 15;319(1-2):45-8. doi: 10.1016/0014-5793(93)80034-r.
A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.
从虹鳟鱼卵巢卵泡cDNA文库中分离出一个编码胆固醇侧链裂解细胞色素P450（P450scc）的cDNA克隆。该cDNA包含一个1542个核苷酸的开放阅读框，编码一个514个氨基酸的蛋白质。虹鳟鱼P450scc的预测氨基酸序列与人类的同源性为48%，与大鼠、牛和猪的同源性为46%。通过用虹鳟鱼P450scc cDNA的表达载体转染COS-1猴肾肿瘤细胞，并随后通过放射免疫测定法检测从25-羟基胆固醇到孕烯醇酮的转化，证实了P450scc的活性。该cDNA仅与一个1.8 kb的RNA转录本杂交。在早期卵黄发生卵泡中未发现该转录本，在卵黄发生后卵泡中几乎未检测到，而在排卵后卵泡中大量存在。
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