Paris N, Robert R, Mercier L
Laboratoire de Biochimie Générale, UFR Sciences de l'Environnement, Université d'Angers, France.
Hybridoma. 1993 Feb;12(1):107-13. doi: 10.1089/hyb.1993.12.107.
Hybridoma cell lines producing monoclonal antibody (Mab) against recombinant human prolactin (rhPrl) were established from fusion between X63-Ag8 myeloma cells and Balb/c mice splenocytes. Four Mabs numbered I to IV were selected by ELISA, purified and characterized. All these Mabs were of the Ig1 kappa isotype and able to recognize oxidized as well as reduced rhPrl. As shown by a competitive inhibition assay, Mab IV did not compete with any of the three others. Moreover, both rhPrl and hPrl extracted from human pituitaries, were recognized equally by this Mab. Properties displayed by Mab IV make it very attractive for the evaluation of prolactin levels by sandwich immunoassays.
通过X63 - Ag8骨髓瘤细胞与Balb/c小鼠脾细胞融合，建立了产生抗重组人催乳素（rhPrl）单克隆抗体（Mab）的杂交瘤细胞系。通过酶联免疫吸附测定（ELISA）选择、纯化并鉴定了4种编号为I至IV的单克隆抗体。所有这些单克隆抗体均为Ig1 κ同种型，能够识别氧化型和还原型rhPrl。竞争性抑制试验表明，单克隆抗体IV与其他三种单克隆抗体中的任何一种均无竞争。此外，该单克隆抗体对rhPrl和从人垂体中提取的hPrl的识别能力相同。单克隆抗体IV所表现出的特性使其在通过夹心免疫测定评估催乳素水平方面极具吸引力。
