小鼠补体第三成分的分离
Isolation of the third component of mouse complement.
作者信息
Gyöngyössy M I, Assimeth S N
出版信息
J Immunol. 1977 Mar;118(3):1032-5.
PMID:845430
Abstract
The third component of mouse complement has been purified to homogeneity from pseudoglobulin by two chromatographic steps involving sequential cation- and anion-exchange chromatography. Mouse C3 is a beta-glycoprotein of m.w. 210,000 consisting of two nonidentical, disulfidelinked polypeptide chains of m.w. 130,000 and 75,000, respectively. The properties of this molecule and its proteolytic fragments closely resemble those of human C3.
摘要
小鼠补体的第三个组分已通过两步色谱法从假球蛋白中纯化至同质，这两步色谱法依次涉及阳离子交换色谱和阴离子交换色谱。小鼠C3是一种分子量为210,000的β-糖蛋白，由两条分子量分别为130,000和75,000的不同的、通过二硫键连接的多肽链组成。该分子及其蛋白水解片段的特性与人类C3的特性极为相似。
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