BonDurant R H, Corbeil R R, Corbeil L B
Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616-8743.
Infect Immun. 1993 Apr;61(4):1385-94. doi: 10.1128/iai.61.4.1385-1394.1993.
Protection by surface antigen TF1.17 of Tritrichomonas foetus was investigated because it reacted with a monoclonal antibody which immobilized and mediated complement killing of the organism and prevented adherence to vaginal epithelial cells. This monoclonal antibody was used to demonstrate conservation of the antigen in most strains and to immunoaffinity purify the 50- to 70-kDa glycoprotein antigen. In preparation for immunization studies, the appropriate challenge dose of parasites was determined by intravaginal inoculation of 23 virgin cows (heifers) with 10(2), 10(4), or 10(6) live organisms at the time of estrus. More animals became infected and vaginal infection was maintained at a higher rate (P < 0.005) over 10 weeks for the group that received 10(6) organisms than in the other two groups. Therefore, this dose was used for challenge of immunized animals. Animals immunized with immunoaffinity-purified TF1.17 antigen in incomplete Freund's adjuvant or incomplete Freund's adjuvant plus dextran sulfate cleared the infection more quickly than adjuvant controls (P < 0.005). Isotype-specific enzyme-linked immunosorbent assay with T. foetus antigen showed that serum immunoglobulin G1 (IgG1) and IgG2 antibody responses as well as cervicovaginal mucus IgG1 and IgA antibodies peaked at about the time of clearance of infection in vaccinated animals. Controls developed later cervicovaginal mucus IgA antibody responses as would be expected in a primary local immune response to infection. These results indicate that vaccination with this immunoaffinity-purified surface antigen of T. foetus enhances antibody responses as well as clearance of the parasite from the female reproductive tract.
对胎儿三毛滴虫表面抗原TF1.17的保护作用进行了研究，因为它能与一种单克隆抗体发生反应，该单克隆抗体可使该生物体固定并介导补体杀伤作用，还能阻止其黏附于阴道上皮细胞。利用这种单克隆抗体证明了大多数菌株中该抗原的保守性，并通过免疫亲和纯化得到了50至70 kDa的糖蛋白抗原。在准备免疫研究时，通过在发情期对23头未孕母牛（小母牛）经阴道接种10²、10⁴或10⁶个活生物体来确定合适的寄生虫攻击剂量。与其他两组相比，接受10⁶个生物体的组中有更多动物被感染，并且在10周内阴道感染维持在更高的发生率（P < 0.005）。因此，该剂量被用于攻击免疫动物。用免疫亲和纯化的TF1.17抗原在不完全弗氏佐剂或不完全弗氏佐剂加硫酸葡聚糖中免疫的动物比佐剂对照组更快清除感染（P < 0.005）。用胎儿三毛滴虫抗原进行的同型特异性酶联免疫吸附试验表明，血清免疫球蛋白G1（IgG1）和IgG2抗体反应以及宫颈阴道黏液IgG1和IgA抗体在接种动物感染清除时达到峰值。对照组宫颈阴道黏液IgA抗体反应出现得较晚，这正如对感染的原发性局部免疫反应所预期的那样。这些结果表明，用这种免疫亲和纯化的胎儿三毛滴虫表面抗原进行疫苗接种可增强抗体反应，并促进寄生虫从雌性生殖道的清除。
