Baba T, Tazaki K, Sonozaki H, Torisu M
J Immunol. 1977 Mar;118(3):762-8.
Neutrophil chemotactic factor(s) and their inhibitors were explored in the acute inflammatory skin lesions induced by the application of 4% DNCB solution with acetone in guinea pigs. Skin biopsies were taken periodically and tissue extracts were made from the biopsy specimens. Neutrophil chemotactic activity found in such extracts reached a peak at 12 to 24 hr after the induction of the inflammation, when the lesions were found to be infiltrated predominantly with neutrophils. After 24 hr, the activity gradually diminished. Physicochemical and antigenic characterization studies on the chemotactic substance indicated that the material was most likely the cleavage product of C3. On the other hand, inhibitors against the neutrophil chemotactic factor were found in the tissue extracts which were obtained from the lesions at a later stage (48 to 96 hr after the induction of the inflammatory reaction). These inhibitors blocked not only the complement-derived chemotactic activity but also that obtained from bacterial culture filtrates. They were heat labile and showed striking heterogeneity in size on Sephadex gel filtration.
在豚鼠中，通过用丙酮涂抹4%二硝基氯苯（DNCB）溶液诱导急性炎症性皮肤损伤，对中性粒细胞趋化因子及其抑制剂进行了研究。定期进行皮肤活检，并从活检标本中制备组织提取物。在诱导炎症后12至24小时，此类提取物中的中性粒细胞趋化活性达到峰值，此时发现损伤部位主要被中性粒细胞浸润。24小时后，活性逐渐降低。对趋化物质的物理化学和抗原特性研究表明，该物质很可能是C3的裂解产物。另一方面，在炎症反应诱导后期（诱导后48至96小时）从损伤部位获得的组织提取物中发现了针对中性粒细胞趋化因子的抑制剂。这些抑制剂不仅阻断了补体衍生的趋化活性，也阻断了从细菌培养滤液中获得的趋化活性。它们对热不稳定，在Sephadex凝胶过滤中显示出大小显著的异质性。
