Kutlar F, Kutlar A, Nuguid E, Prchal J, Huisman T H
Department of Biochemistry, Medical College of Georgia, Augusta 30912-2100.
Hemoglobin. 1993 Feb;17(1):55-66. doi: 10.3109/03630269308998885.
We have analyzed the blood samples from seven members of two families that contained mixtures of different hemoglobin types due to the simultaneous presence of different beta chain variants and the alpha chain variant Hb G-Philadelphia. The methods used were isoelectrofocusing and cation exchange high performance liquid chromatography for the separation of the hemoglobin components, and reversed phase high performance liquid chromatography for that of the various alpha and beta chains; the chromatographic procedures also provided quantitative data. Application of these fast and simple high performance liquid chromatographic methods greatly aided in defining the different hemoglobinopathies that were present in these persons, including an additional alpha-thalassemia-2 heterozygosity or homozygosity.
我们分析了来自两个家族七名成员的血样，这些血样因同时存在不同的β链变异体和α链变异体Hb G - 费城而含有不同血红蛋白类型的混合物。所使用的方法包括等电聚焦和阳离子交换高效液相色谱法用于分离血红蛋白成分，反相高效液相色谱法用于分离各种α链和β链；色谱程序还提供了定量数据。这些快速且简单的高效液相色谱方法的应用极大地有助于确定这些人所患的不同血红蛋白病，包括额外的α地中海贫血-2杂合性或纯合性。
