Wadhwa R, Kaul S C, Ikawa Y, Sugimoto Y
Aging Process Research Laboratory, Tsukuba Life Science Center, Ibaraki, Japan.
J Biol Chem. 1993 Mar 25;268(9):6615-21.
A novel 66-kDa protein, named p66mot-1, is identified to be associated with cellular mortality by virtue of its presence in cytosolic fractions of serially passaged mouse embryonic fibroblasts (MEF) and the mortal hybrids obtained by the fusion of mortal (MEF) and immortal (MN48-1, derivative of NIH 3T3) cells. Immortal cells lack this protein in their cytosolic fractions. cDNA cloning and homology search placed it in the heat shock protein 70 (hsp70) family. Microinjection of anti-p66 antibody to senescent MEF could transiently stimulate their cell division, which supports the cellular mortality-related function of p66mot-1.
一种名为p66mot-1的新型66 kDa蛋白，因其存在于连续传代的小鼠胚胎成纤维细胞（MEF）的胞质组分以及由死亡的（MEF）和永生的（MN48-1，NIH 3T3的衍生物）细胞融合得到的死亡杂种细胞中，而被鉴定与细胞死亡率相关。永生细胞的胞质组分中缺乏这种蛋白质。cDNA克隆和同源性搜索将其归入热休克蛋白70（hsp70）家族。向衰老的MEF显微注射抗p66抗体可短暂刺激其细胞分裂，这支持了p66mot-1与细胞死亡率相关的功能。
