Nyyssönen K, Seppänen K, Salonen J T
Research Institute of Public Health, University of Kuopio, Finland.
J Chromatogr. 1993 Jan 29;612(1):27-32. doi: 10.1016/0378-4347(93)80363-9.
A method for the routine determination of platelet-produced thromboxane B2 (TXB2) from human serum is presented. To induce the secretion of thromboxane A2 from the platelets, blood is kept at 37 degrees C for 30 min before serum is separated. Serum is prepurified through small reversed-phase columns and TXB2 is analysed by reversed-phase high-performance liquid chromatography. A column-switching technique is used to remove the interfering compounds present in serum. The detection limit with standard solution is 30 ng per injection. The method was applied to the measurement of platelet-produced TXB2 serum from 1040 men. The mean TXB2 was 247 +/- 134 ng/ml in the serum of men who had not used prostaglandin inhibitors, and 208 +/- 123 ng/ml in the serum of men who had used a prostaglandin inhibitor during a two-week period before blood sampling.
本文介绍了一种从人血清中常规测定血小板产生的血栓素B2（TXB2）的方法。为诱导血小板分泌血栓素A2，在分离血清前将血液于37℃保存30分钟。血清通过小型反相柱进行预纯化，TXB2采用反相高效液相色谱法进行分析。使用柱切换技术去除血清中存在的干扰化合物。标准溶液的检测限为每次进样30 ng。该方法应用于1040名男性血清中血小板产生的TXB2的测定。未使用前列腺素抑制剂的男性血清中TXB2的平均值为247±134 ng/ml，在采血前两周内使用过前列腺素抑制剂的男性血清中TXB2的平均值为208±123 ng/ml。
