Kizaki T, Ishige M, Bingyan W, Kumagai M, Day N K, Good R A, Onoé K
Hokkaido Institute of Public Health, Japan.
J Leukoc Biol. 1993 Mar;53(3):233-9. doi: 10.1002/jlb.53.3.233.
Mitogenic effects of protoscoleces (PSCs) of Echinococcus multilocularis on murine lymphocytes were studied. Spleen cells from normal BALB/c mice showed significant proliferative responses when cocultured with PSCs. Proliferative responses were observed in both the T and B cell populations. The PSCs also stimulated cells of the macrophage/monocyte lineage to secrete interleukin-1 (IL-1). Depletion of plastic- and Sephadex G-10-adherent cells from the spleen cell population significantly reduced the proliferative responses to PSCs and the low responsiveness was restored by addition of plastic-adherent cells to these cultures. Furthermore, addition of anti-IL-1 serum to the spleen cell cultures stimulated with PSCs completely suppressed the proliferative responses. These findings demonstrate that the mitogenic effect of PSC on lymphocytes depends on IL-1 secreted by cells of macrophage/monocyte lineage.
研究了多房棘球绦虫原头节（PSCs）对小鼠淋巴细胞的促有丝分裂作用。正常BALB/c小鼠的脾细胞与PSCs共培养时显示出显著的增殖反应。在T细胞和B细胞群体中均观察到增殖反应。PSCs还刺激巨噬细胞/单核细胞系的细胞分泌白细胞介素-1（IL-1）。从脾细胞群体中去除贴壁于塑料和葡聚糖凝胶G-10的细胞可显著降低对PSCs的增殖反应，而向这些培养物中添加贴壁于塑料的细胞可恢复低反应性。此外，向用PSCs刺激的脾细胞培养物中添加抗IL-1血清可完全抑制增殖反应。这些发现表明，PSC对淋巴细胞的促有丝分裂作用取决于巨噬细胞/单核细胞系细胞分泌的IL-1。
