Tseng Y C, Kim J, Park K
Purdue University, School of Pharmacy, West Lafayette, IN 47907.
J Biomater Appl. 1993 Jan;7(3):233-49. doi: 10.1177/088532829300700303.
4-Azido-2-nitrophenyl albumin (ANP-albumin) was prepared by displacing the fluoro group of 4-fluoro-3-nitrophenyl azide (FNPA) by an amino group of albumin. Photolysis of phenyl azides of ANP-albumin was studied by Fourier-transform infrared (FTIR) spectroscopy. The band of phenyl azide disappeared completely after a 12-min exposure to long wave UV light (366 nm), and the photolysis was first-order. Albumin was grafted onto dimethyldichlorosilane-coated glass (DDS-glass) by photolysis of the azido groups of ANP-albumin without any premodification of the surface. The albumin-grafted DDS-glass was characterized by determining the relative amount of nitrogen resulting from the grafted albumin on the surface using electron spectroscopy for chemical analysis (ESCA). The amount of nitrogen increased when the concentration of ANP-albumin in the adsorption solution increased up to 0.1 mg/ml. As the solution concentration increased above this value, the amount of nitrogen decreased. The platelet resistance of the albumin-grafted surfaces was evaluated by measuring the number of adherent platelets and the extent of activation that was quantitated by the area of platelets spread on the surfaces. The maximum platelet-resistant effect was observed when the ANP-albumin was adsorbed for more than 50 min at the solution concentration ranging from 0.05 to 10 mg/ml.
4-叠氮基-2-硝基苯基白蛋白（ANP-白蛋白）是通过白蛋白的氨基取代4-氟-3-硝基苯基叠氮化物（FNPA）的氟基团制备而成。利用傅里叶变换红外（FTIR）光谱研究了ANP-白蛋白中苯基叠氮化物的光解。在暴露于长波紫外光（366 nm）12分钟后，苯基叠氮化物的谱带完全消失，且光解为一级反应。在未对表面进行任何预改性的情况下，通过ANP-白蛋白叠氮基团的光解将白蛋白接枝到二甲基二氯硅烷涂层玻璃（DDS-玻璃）上。通过使用化学分析电子能谱（ESCA）测定表面接枝白蛋白产生的氮的相对含量，对白蛋白接枝的DDS-玻璃进行了表征。当吸附溶液中ANP-白蛋白的浓度增加至0.1 mg/ml时，氮含量增加。当溶液浓度高于此值时，氮含量降低。通过测量粘附血小板的数量以及通过血小板在表面铺展面积定量的活化程度，评估了白蛋白接枝表面的抗血小板性能。当在0.05至10 mg/ml的溶液浓度下，ANP-白蛋白吸附超过50分钟时，观察到最大的抗血小板效果。
