Booth J E
J Endocrinol. 1977 Feb;72(2):135-41. doi: 10.1677/joe.0.0720135.
Male rats were castrated on the day of birth (day 1) and injected with either testosterone, dihydrotestosterone, a synthetic oestrogen (RU 2858 + dihydrotestosterone, or oil from days 1 to 5. The aromatizable androgen, testosterone, and RU 2858 suppressed both cyclic gonadotrophin secretion, indicated by the absence of corpora lutea from implanted ovarian grafts, and the behavioural response to oestradiol benzoate + progesterone injections in adulthood. The 5alpha-reduced androgen, dihydrotestosterone alone did not affect gonadotrophin secretion or female receptive behaviour, but like testosterone, it increased penis development in response to testosterone propionate, and this was positively correlated with copulatory efficiency, i.e. the ratio of intromission to mount frequencies. Nevertheless, ejaculation only occurred among animals that had received testosterone or RU 2858 + dihydrotestosterone. The results support the concept that during the preinatal period, neural conversion of androgens to oestrogens is important both for the suppression of female gonadotrophin secretion and behaviour patterns as well as for the organization of male behaviour patterns. The 5alpha-reduction of unsaturated C19-steriods to dihydrotestosterone in peripheral tissues is also required to complete the development of the male genital tract.
雄性大鼠在出生当天（第1天）进行去势，并从第1天至第5天注射睾酮、双氢睾酮、一种合成雌激素（RU 2858 + 双氢睾酮）或油。可芳香化的雄激素睾酮和RU 2858抑制了周期性促性腺激素的分泌，这表现为植入的卵巢移植物中没有黄体，以及成年后对苯甲酸雌二醇 + 孕酮注射的行为反应。5α - 还原雄激素双氢睾酮单独并不影响促性腺激素的分泌或雌性接受行为，但与睾酮一样，它会因丙酸睾酮而增加阴茎发育，且这与交配效率呈正相关，即插入频率与爬跨频率的比值。然而，射精仅发生在接受睾酮或RU 2858 + 双氢睾酮的动物中。这些结果支持了这样一种概念，即在出生前时期，雄激素向雌激素的神经转化对于抑制雌性促性腺激素分泌和行为模式以及对于雄性行为模式的组织都很重要。外周组织中不饱和C19 - 甾体向双氢睾酮的5α - 还原对于雄性生殖道的完全发育也是必需的。
