Sudol M, Greulich H, Newman L, Sarkar A, Sukegawa J, Yamamoto T
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Oncogene. 1993 Apr;8(4):823-31.
While screening a chicken kidney cDNA library for the normal homolog of the yes oncogene, we isolated a clone that encodes a novel non-receptor type protein tyrosine kinase of the Src family. We named this gene product Yrk (York), as an acronym for Yes-related kinase. As predicted from the cDNA sequence, the Yrk protein consists of 536 amino acids and has all the canonical features of a Src kinase. At the amino terminus it contains a myristylation signal, followed by a unique domain, SH3 and SH2 motifs, an ATP binding site, a kinase region and a carboxy-terminal sequence with a potential regulatory tyrosine at position 530. The sequence of the Yrk protein showed 79% identity with human Fyn and 72% identity with chicken Yes. To eliminate the possibility that the Yrk protein is an avian homolog of mammalian Fyn, we isolated and sequenced the chicken fyn cDNA. The sequence data together with Southern and Northern blot analyses showed that the chicken yrk gene is distinct from the chicken fyn gene. Antibodies generated against the unique domain of the yrk protein expressed in bacteria precipitated a 60-kDa protein that was active in an immune complex kinase assay and was phosphorylated on tyrosine. Expression of the Yrk protein in adult chicken tissues was elevated in cerebellum and spleen. Relatively high levels of Yrk were also found in lung and skin.
在筛选鸡肾cDNA文库以寻找yes癌基因的正常同源物时，我们分离出一个克隆，它编码一种Src家族的新型非受体型蛋白酪氨酸激酶。我们将这个基因产物命名为Yrk（York），作为Yes相关激酶的首字母缩写。根据cDNA序列预测，Yrk蛋白由536个氨基酸组成，具有Src激酶的所有典型特征。在氨基末端，它含有一个肉豆蔻酰化信号，接着是一个独特结构域、SH3和SH2基序、一个ATP结合位点、一个激酶区域以及一个在第530位带有潜在调节性酪氨酸的羧基末端序列。Yrk蛋白的序列与人类Fyn的同一性为79%，与鸡Yes的同一性为72%。为了排除Yrk蛋白是哺乳动物Fyn的禽类同源物的可能性，我们分离并测序了鸡fyn cDNA。序列数据以及Southern和Northern印迹分析表明，鸡yrk基因与鸡fyn基因不同。针对在细菌中表达的yrk蛋白独特结构域产生的抗体沉淀出一种60 kDa的蛋白，该蛋白在免疫复合物激酶测定中具有活性并且在酪氨酸上被磷酸化。Yrk蛋白在成年鸡组织中的表达在小脑和脾脏中升高。在肺和皮肤中也发现了相对较高水平的Yrk。
