Huang S, Hendriks W, Althage A, Hemmi S, Bluethmann H, Kamijo R, Vilcek J, Zinkernagel R M, Aguet M
Institute of Molecular Biology I, University of Zürich, Switzerland.
Science. 1993 Mar 19;259(5102):1742-5. doi: 10.1126/science.8456301.
Interferon-gamma (IFN-gamma) exerts pleiotropic effects, including antiviral activity, stimulation of macrophages and natural killer cells, and increased expression of major histocompatibility complex antigens. Mice without the IFN-gamma receptor had no overt anomalies, and their immune system appeared to develop normally. However, mutant mice had a defective natural resistance, they had increased susceptibility to infection by Listeria monocytogenes and vaccinia virus despite normal cytotoxic and T helper cell responses. Immunoglobulin isotype analysis revealed that IFN-gamma is necessary for a normal antigen-specific immunoglobulin G2a response. These mutant mice offer the possibility for the further elucidation of IFN-gamma-mediated functions by transgenic cell- or tissue-specific reconstitution of a functional receptor.
γ干扰素（IFN-γ）具有多种效应，包括抗病毒活性、刺激巨噬细胞和自然杀伤细胞以及增加主要组织相容性复合体抗原的表达。缺乏IFN-γ受体的小鼠没有明显异常，其免疫系统似乎发育正常。然而，突变小鼠的天然抵抗力存在缺陷，尽管其细胞毒性和T辅助细胞反应正常，但它们对单核细胞增生李斯特菌和痘苗病毒感染的易感性增加。免疫球蛋白同种型分析显示，IFN-γ对于正常的抗原特异性免疫球蛋白G2a反应是必需的。这些突变小鼠为通过转基因细胞或组织特异性重建功能性受体进一步阐明IFN-γ介导的功能提供了可能性。
