使用免疫吸附结合生物测定法生产抗艰难梭菌细胞毒素的单克隆抗体。

Production of monoclonal antibodies against Clostridium difficile cytotoxin using immunosorbent binding bioassay procedure.

作者信息

Depitre C, L'Haridon R M, Lyerly D M, Barroso L A, Wilkins T D, Andremont A, Corthier G

机构信息

Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy, Jouy-en-Josas, France.

出版信息

Toxicon. 1993 Feb;31(2):181-6. doi: 10.1016/0041-0101(93)90284-p.

DOI:10.1016/0041-0101(93)90284-p

PMID:8456445

Abstract

In the following study, a novel screening approach was used to develop monoclonal antibodies specific for toxin B of Clostridium difficile. The approach, which consisted of an immunosorbent binding bioassay (ISBBA), is based on antigen immunocapture by monoclonal antibodies and detection of biological activity. Our results showed ISBBA, which uses unpurified antigen, to be more sensitive than the neutralization assay and ELISA for the detection of toxin B antibody.

摘要

在以下研究中，采用了一种新型筛选方法来开发针对艰难梭菌毒素B的特异性单克隆抗体。该方法由免疫吸附结合生物测定法（ISBBA）组成，基于单克隆抗体对抗原的免疫捕获和生物活性检测。我们的结果表明，使用未纯化抗原的ISBBA在检测毒素B抗体方面比中和试验和酶联免疫吸附测定（ELISA）更敏感。