Atmani F, Lacour B, Drüeke T, Daudon M
Département de Néphrologie, Hôpital-Necker Enfants Malades, Paris, France.
Urol Res. 1993 Jan;21(1):61-6. doi: 10.1007/BF00295195.
A calcium oxalate crystal growth inhibitor was isolated from human urine using DEAE-Sephacel gel followed by Sephacryl S-300 chromatography and FPLC column. The isolated inhibitor was a uronic-acid-rich protein (UAP). It was found to be a glycoprotein with a molecular weight of 35,000 Da as determined by SDS-polyacrylamide gel electrophoresis. Inhibitory activity was demonstrated using a calcium oxalate crystallization system. In addition UAP, nephrocalcin (NC) or nephrocalcin-like (NC-like) activity was an effective inhibitor in this system. However, the inhibitory activity of UAP appeared to be higher than that of NC or NC-like activity. This finding suggests that NC or NC-like activity is not only urinary protein with strong inhibitory activity. UAP and probably other proteins also play a role in the control of urinary crystal growth.
采用DEAE-葡聚糖凝胶，随后通过Sephacryl S-300色谱法和快速蛋白质液相色谱柱，从人尿液中分离出一种草酸钙晶体生长抑制剂。分离出的抑制剂是一种富含糖醛酸的蛋白质（UAP）。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，发现它是一种分子量为35,000道尔顿的糖蛋白。使用草酸钙结晶系统证明了其抑制活性。此外，UAP、肾钙素（NC）或类肾钙素（NC样）活性在该系统中是一种有效的抑制剂。然而，UAP的抑制活性似乎高于NC或NC样活性。这一发现表明，NC或NC样活性并非唯一具有强抑制活性的尿蛋白。UAP以及可能的其他蛋白质在控制尿晶体生长中也发挥作用。
