采用FETAX程序测定Cu2+和Zn2+对非洲爪蟾的胚胎毒性和致畸性。

Embryotoxicity and teratogenicity of Cu2+ and Zn2+ for Xenopus laevis, assayed by the FETAX procedure.

作者信息

Luo S Q, Plowman M C, Hopfer S M, Sunderman F W

机构信息

Department of Laboratory Medicine, University of Connecticut School of Medicine, Farmington 06030.

出版信息

Ann Clin Lab Sci. 1993 Mar-Apr;23(2):111-20.

PMID:8457140

Abstract

Cupric chloride (CuCl2) and zinc chloride (ZnCl2) were tested by the FETAX (Frog Embryo Teratogenesis Assay: Xenopus) procedure in the South African frog, Xenopus laevis. The median teratogenic concentrations (EC50) of Cu2+ and Zn2+ were 2.5 and 40 mumol per L. The median embryolethal concentrations (LC50) of Cu2+ and Zn2+ were 22 and 850 mumol per L. The teratogenic indices (TI = LC50/EC50) were 8.8 for Cu2+ and 21 for Zn2+. Both metal ions were shown to be potent teratogens for Xenopus, causing concentration-related increases of eye, gut, facial, notochord, and cardiac anomalies.

摘要

通过南非爪蟾（非洲爪蟾）的FETAX（蛙胚胎致畸试验：非洲爪蟾）程序对氯化铜（CuCl₂）和氯化锌（ZnCl₂）进行了测试。Cu²⁺和Zn²⁺的致畸中浓度（EC₅₀）分别为每升2.5微摩尔和40微摩尔。Cu²⁺和Zn²⁺的胚胎致死中浓度（LC₅₀）分别为每升22微摩尔和850微摩尔。致畸指数（TI = LC₅₀/EC₅₀），Cu²⁺为8.8，Zn²⁺为21。两种金属离子对非洲爪蟾均显示为强效致畸剂，会导致眼睛、肠道、面部、脊索和心脏异常随浓度增加。