Spiller H, Stallings W
Department of Biology, Fisk University, Nashville, TN 37208.
Biotechniques. 1993 Mar;14(3):420-2.
This report describes a reliable method of 14CO2 recovery from 14C-labeled sucrose applied to leaves of young wheat plants. After completion of the labeling experiment, sections of the plant are harvested, digested in concentrated sulfuric acid and oxidized by 3% hydrogen peroxide in a closed system containing CO2-absorbing 2 N NaOH. Heating the sulfuric acid containing vial to 85 degrees C is necessary to achieve near total recovery of 14C-label within 30 min. This technique can be applied to other plant or animal systems, to study the translocation of organic 14C-label.
