Bird I F, Cornelius M J, Keys A J, Whittingham C P
Biochem J. 1978 Apr 15;172(1):23-7. doi: 10.1042/bj1720023.
Pea leaves were illuminated in air containing 150 or 1000p.p.m. of 14CO2 for various times. Alternatively, segments of wheat leaves were supplied with [3-14C]serine for 40 min in the light in air with 145, 326 or 944p.p.m. of 12CO2. Sucrose was extracted from the leaf material, hydrolysed with invertase, and 14C in the pairs of carbon atoms C-3+C-4, C-2+C-5 and C-1+C-6 in the glucose moiety was measured. The results obtained after metabolism of 14CO2 were consistent with the operation of the photosynthetic carbon-reduction cycle; the effects of CO2 concentration on distribution of 14C in the carbon chain of glucose after metabolism of [3-14C]serine is more easily explained by metabolism through the glycollate pathway than by the carbon-reduction cycle.
豌豆叶片在含有150或1000ppm 14CO2的空气中光照不同时间。或者，在含有145、326或944ppm 12CO2的空气中，小麦叶片切段在光照下用[3-14C]丝氨酸供应40分钟。从叶片材料中提取蔗糖，用转化酶水解，并测量葡萄糖部分中C-3 + C-4、C-2 + C-5和C-1 + C-6碳原子对中的14C。14CO2代谢后获得的结果与光合碳还原循环的运行一致；[3-14C]丝氨酸代谢后，CO2浓度对葡萄糖碳链中14C分布的影响，通过乙醇酸途径代谢比通过碳还原循环更容易解释。
