Gray D F, Eastwood M A, Brydon W G, Fry S C
Department of Medicine, Western General Hospital, Edinburgh.
Br J Nutr. 1993 Jan;69(1):189-97. doi: 10.1079/bjn19930021.
A 14C-labelled plant cell wall preparation (14C-PCW) produced from spinach (Spinacia oleracea L.) cell culture exhibits uniform labelling of the major polysaccharide groups (%): pectins 53, hemicellulose 13, cellulose 21, starch 3. This 14C-PCW preparation has been used in rat studies as a marker for plant cell wall metabolism. Metabolism of the 14C-PCW occurred largely over the first 24 h. This was due to fermentation in the caecum. The pectic fraction of the plant cell walls was degraded completely in the rat gastrointestinal tract, but some [14C]cellulose was still detected after 24 h in the colon. Of the 14C, 22% was recovered in the host liver, adipose tissue and skin, 26% excreted as 14CO2 and up to 18% was excreted in the faeces. There was no urinary excretion of 14C. In vitro fermentation using a caecal inoculum showed reduced 14CO2 production, 12% compared with 26% in the intact rat. 14C-PCW is a useful marker to investigate the fate of plant cell wall materials in the gastrointestinal tract. These studies show both bacterial fermentation of the 14C-PCW and host metabolism of the 14C-labelled fermentation products.
一种由菠菜（Spinacia oleracea L.）细胞培养物制备的14C标记的植物细胞壁制剂（14C-PCW），其主要多糖基团呈现均匀标记（百分比）：果胶53、半纤维素13、纤维素21、淀粉3。这种14C-PCW制剂已用于大鼠研究，作为植物细胞壁代谢的标记物。14C-PCW的代谢主要发生在最初的24小时内。这是由于盲肠中的发酵作用。植物细胞壁的果胶部分在大鼠胃肠道中被完全降解，但在结肠中24小时后仍能检测到一些[14C]纤维素。在14C中，22%在宿主肝脏、脂肪组织和皮肤中回收，26%以14CO2形式排出，高达18%通过粪便排出。没有14C通过尿液排出。使用盲肠接种物进行的体外发酵显示14CO2产量降低，完整大鼠中为26%，而体外发酵为12%。14C-PCW是研究植物细胞壁物质在胃肠道中归宿的有用标记物。这些研究表明了14C-PCW的细菌发酵以及14C标记的发酵产物的宿主代谢。
