Sse8647I，一种来自链霉菌属的新型II型限制性内切酶，切割位点为5'-AG/GWCCT-3'。
Sse8647I, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'.
作者信息
Ishino Y, Nomura Y, Kato I
机构信息
Biotechnology Research Laboratories, Takara Shuzo Co. Ltd, Shiga, Japan.
出版信息
Nucleic Acids Res. 1995 Mar 11;23(5):742-4. doi: 10.1093/nar/23.5.742.
Abstract
We isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Streptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave lambda phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and phi X174 DNAs.
摘要
我们从链霉菌属的一种微生物中分离并鉴定出一种新型II型限制性内切酶，该酶识别回文七核苷酸序列5'-AGGWCCT-3'，并在该序列中的第一个G后切割双链DNA。这种酶在八个位点切割腺病毒2 DNA，但不切割λ噬菌体、pBR322、pUC18和19、M13mp18和19、SV40、ColE1和φX174 DNA。
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