Sse8647I, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'.
作者信息
Ishino Y, Nomura Y, Kato I
机构信息
Biotechnology Research Laboratories, Takara Shuzo Co. Ltd, Shiga, Japan.
出版信息
Nucleic Acids Res. 1995 Mar 11;23(5):742-4. doi: 10.1093/nar/23.5.742.
Abstract
We isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Streptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave lambda phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and phi X174 DNAs.
摘要
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