Holter W, Kalthoff F S, Majdic O, Knapp W
Institute of Immunology, University of Vienna, Austria.
Lymphokine Cytokine Res. 1993 Feb;12(1):33-8.
Interleukin-4 (IL-4) is a cytokine difficult to induce in large quantities in freshly isolated cells. No malignant cell line has been described to date to produce IL-4 either constitutively or following activation. Here we report that HUT78 cells can be induced to produce IL-4 either by CD3 stimulation under cross-linking conditions or by soluble CD3 mAbs in the presence of PMA. IL-4-specific mRNA could be demonstrated readily under such conditions. Using this experimental model we investigated the role of IL-2 for IL-4 production. The addition of IL-2 strongly costimulated CD3-induced IL-4 production, whereas blockade of the IL-2/IL-2R pathway reduced IL-4 production by approximately 50%. IL-2 alone, however, could not induce IL-4 production. These results indicate that IL-2 is a helper of cytokine necessary, but not sufficient, for IL-4 production in HUT78 cells.
白细胞介素-4（IL-4）是一种在新鲜分离的细胞中难以大量诱导产生的细胞因子。迄今为止，尚未有描述任何恶性细胞系能组成性地或在激活后产生IL-4。在此我们报告，HUT78细胞在交联条件下通过CD3刺激或在PMA存在时通过可溶性CD3单克隆抗体可被诱导产生IL-4。在这些条件下可轻易证实IL-4特异性mRNA的存在。利用这个实验模型，我们研究了IL-2对IL-4产生的作用。添加IL-2强烈共刺激CD3诱导的IL-4产生，而阻断IL-2/IL-2R途径可使IL-4产生减少约50%。然而，单独的IL-2不能诱导IL-4产生。这些结果表明，IL-2是HUT78细胞中IL-4产生所必需的细胞因子辅助因子，但并不充分。
