Spannagl M, Trauner A, Birg A, Frank G, Hoffmann H, Siebeck M, Lill H
Department of Medicine, Klinikum Innenstadt, University of Munich, Germany.
Blood Coagul Fibrinolysis. 1993 Feb;4(1):103-6.
Elevated levels of soluble fibrin in plasma indicate that thrombin converts fibrinogen to fibrin without sufficient inhibitory control. Therefore, measurement of soluble fibrin (SF) in plasma may be considered as a laboratory test for intravascular coagulation. We have demonstrated that a new immunoassay for detection of SF in human plasma (Lill et al., Blood Coag Fibrinol 1993; 4: 97-102), based on a fibrin specific monoclonal antibody, also detects porcine SF with high sensitivity. Thrombin-dependent generation of SF in porcine plasma in vitro resulted in increased reactivity of the assay system, which was time and dose dependent. Dextran sulphate (DXS) and bacterial lipopolysaccharide (LPS) were used as stimuli in in vivo experiments in pigs. Plasma levels of SF increased steadily after intravenous administration of DXS (5 mg/kg for 1 h) to 38 +/- 7.8 micrograms/ml (mean +/- SEM) at 2 h, whereas LPS (2 micrograms/kg/h for 6 h) markedly increased plasma SF levels to over 120 micrograms/ml (at 6 h) after a lag phase of 2 h. In conclusion, this new immunoassay for human fibrin allows specific and sensitive detection of soluble fibrin in porcine plasma.
血浆中可溶性纤维蛋白水平升高表明凝血酶在没有足够抑制控制的情况下将纤维蛋白原转化为纤维蛋白。因此，血浆中可溶性纤维蛋白（SF）的测定可被视为血管内凝血的一项实验室检测。我们已经证明，一种基于纤维蛋白特异性单克隆抗体检测人血浆中SF的新免疫测定法（Lill等人，《血液凝固与纤维蛋白溶解》1993年；4：97 - 102），也能高灵敏度地检测猪的SF。体外猪血浆中凝血酶依赖性SF的生成导致测定系统的反应性增加，这是时间和剂量依赖性的。硫酸葡聚糖（DXS）和细菌脂多糖（LPS）在猪的体内实验中用作刺激物。静脉注射DXS（5毫克/千克，持续1小时）后，血浆SF水平在2小时时稳定升高至38±7.8微克/毫升（平均值±标准误），而LPS（2微克/千克/小时，持续6小时）在2小时的滞后阶段后，在6小时时将血浆SF水平显著提高至超过120微克/毫升。总之，这种用于人纤维蛋白的新免疫测定法能够特异性且灵敏地检测猪血浆中的可溶性纤维蛋白。
