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人转铁蛋白聚集体的X射线小角散射。一项分形研究。

X-ray small angle scattering of the human transferrin protein aggregates. A fractal study.

作者信息

Castellano A C, Barteri M, Bianconi A, Borghi E, Cassiano L, Castagnola M, Della Longa S

机构信息

Dipartimento di Fisica Universita La Sapienza, Roma, Italy.

出版信息

Biophys J. 1993 Feb;64(2):520-4. doi: 10.1016/S0006-3495(93)81394-8.

Abstract

X-ray small angle scattering experiments, using a pin hole SAXS camera with Synchrotron radiation source, have been performed to study the conformational changes of lyophilized samples of Apo-, Mono-, and Diferric- human transferrin. We report the experimental evidence that the analysis of the scattered intensity through the fractal theory may give information on the particle size and its variation upon iron binding.

摘要

利用带有同步辐射源的针孔小角X射线散射相机进行了X射线小角散射实验,以研究脱铁、单铁和双铁人转铁蛋白冻干样品的构象变化。我们报告了实验证据,即通过分形理论对散射强度进行分析可以提供有关颗粒大小及其在铁结合时变化的信息。

相似文献

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Metal-induced conformational changes in transferrins.金属诱导的转铁蛋白构象变化。
J Mol Biol. 1993 Feb 5;229(3):585-90. doi: 10.1006/jmbi.1993.1063.

本文引用的文献

3
Size and shape determination of apotransferrin and transferrin monomers.
Biopolymers. 1971 Jun;10(6):1039-48. doi: 10.1002/bip.360100610.
5
Fractal surfaces of proteins.蛋白质的分形表面
Science. 1985 Dec 6;230(4730):1163-5. doi: 10.1126/science.4071040.
7
Molecular structure of serum transferrin at 3.3-A resolution.
Biochemistry. 1988 Jul 26;27(15):5804-12. doi: 10.1021/bi00415a061.

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