Malbica J O, Monson K, Neilson K, Sprissler R
J Pharm Sci. 1977 Mar;66(3):384-6. doi: 10.1002/jps.2600660318.
A GLC method for the determination of plasma isosorbide dinitrate using electron-capture detection is described. The organic nitrates are especially suited for electron-capture detection if the detector temperature is optimized for maximum sensitivity, e.g., 175 degrees. Proper maintenance of the detector and column assures reproducible data in the low nanogram range. The extraction procedure described is simple, efficient, and expedient for processing large numbers of samples. The method was used to study plasma levels in four human volunteers after a single dose of a 5-mg chewable isosorbide dinitrate tablet. Concentration levels of isosorbide dinitrate as low as 0.5 ng/ml of plasma can be measured by this procedure.
描述了一种使用电子捕获检测法测定血浆中硝酸异山梨酯的气相色谱法。如果将检测器温度优化至最大灵敏度，例如175摄氏度，有机硝酸盐特别适合电子捕获检测。正确维护检测器和色谱柱可确保在低纳克范围内获得可重复的数据。所描述的提取程序简单、高效且便于处理大量样品。该方法用于研究四名人类志愿者单次服用5毫克咀嚼片硝酸异山梨酯后的血浆水平。通过该程序可测量低至0.5纳克/毫升血浆的硝酸异山梨酯浓度水平。
