Chin D A, Prue D G, Michelucci J, Kho B T, Warner C R
J Pharm Sci. 1977 Aug;66(8):1143-5. doi: 10.1002/jps.2600660823.
A GLC method for the determination of plasma isosorbide dinitrate and two metabolites, isosorbide 2-mononitrate and isosorbide 5-mononitrate, is described. The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electron-capture detection after GLC separation with a 30% SE-30 column. The unusually heavy liquid phase loading is necessary to eliminate irreversible adsorption on the solid support. The electron-capture detector provides excellent sensitivity and specificity because of the electronegative nature of the nitric ester. The method was used to study the blood levels of isosorbide dinitrate and two metabolites in four beagle dogs after single oral doses of 40 mg of isosorbide dinitrate and in two human volunteers after a sublingual dose of 10 mg of isosorbide dinitrate.
本文描述了一种用于测定血浆中硝酸异山梨酯及其两种代谢产物 2-单硝酸异山梨酯和 5-单硝酸异山梨酯的气相色谱法。这三种物质从碱化血浆中用乙醚萃取。通过在 30% SE - 30 柱上进行气相色谱分离后，采用电子捕获检测进行定量。需要异常高的液相负载量以消除在固体载体上的不可逆吸附。由于硝酸酯的电负性，电子捕获检测器具有出色的灵敏度和特异性。该方法用于研究 4 只比格犬单次口服 40mg 硝酸异山梨酯后以及 2 名人类志愿者舌下含服 10mg 硝酸异山梨酯后血浆中硝酸异山梨酯及其两种代谢产物的血药浓度。
