Lawin-Brüssel C A, Refojo M F, Leong F L, Hanninen L, Kenyon K R
Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Cornea. 1993 Jan;12(1):10-8. doi: 10.1097/00003226-199301000-00003.
Pseudomonas aeruginosa adherence in vitro to perfilcon A (ionic, 71% H2O) extended wear soft contact lenses--both new and after 7 days of continuous wear on closed rabbit eyes--was found to be related directly to the bacterial concentration in the contaminating solution. Thirty rabbits wore perfilcon A lenses for 7 days with complete lid closure to mimic contact lens overwear. After 7 days, conjunctival cultures showed no growth of pathogens, but all corneas had developed epithelial cell exfoliation and/or epithelial defects and stromal edema. The lenses were then incubated in various concentrations (10(7), 10(6), 10(5), 10(4), and 10(2) colony-forming units per milliliter or saline control; n = 5/group) of P. aeruginosa suspensions and replaced on their respective corneas with tarsorrhaphies for an additional 48 h. By day 9, corneal thickness had increased significantly, and P. aeruginosa keratitis had developed in 13 of 25 bacterially exposed eyes but not in 5 control eyes. Although with decreasing P. aeruginosa concentration the prevalence of ulcerative microbial keratitis also decreased, the initial concentration of bacteria or the initial extent of soft contact lens-induced corneal damage had no influence on the ultimate clinical severity of the disease.
研究发现，铜绿假单胞菌在体外对亲水性丙烯酸酯（离子型，含水量71%）软性角膜接触镜（无论是新镜片还是在兔眼闭合状态下连续佩戴7天后的镜片）的黏附，与污染溶液中的细菌浓度直接相关。30只兔子佩戴亲水性丙烯酸酯镜片7天，完全闭合眼睑以模拟隐形眼镜过度佩戴。7天后，结膜培养显示无病原体生长，但所有角膜均出现上皮细胞剥脱和/或上皮缺损以及基质水肿。然后将镜片置于不同浓度（每毫升10⁷、10⁶、10⁵、10⁴和10²菌落形成单位或生理盐水对照；每组n = 5）的铜绿假单胞菌悬液中孵育，并通过睑裂缝合将其重新置于各自的角膜上，再持续48小时。到第9天，角膜厚度显著增加，25只暴露于细菌的眼中有13只发生了铜绿假单胞菌性角膜炎，而5只对照眼中未发生。虽然随着铜绿假单胞菌浓度降低，溃疡性微生物角膜炎的患病率也降低，但细菌的初始浓度或软性角膜接触镜诱导的角膜损伤的初始程度对疾病最终的临床严重程度没有影响。
