Thompson K P, Hanna K D, Gipson I K, Gravagna P, Waring G O, Johnson-Wint B
Department of Ophthalmology, Emory University, Atlanta, Georgia 30329.
Cornea. 1993 Jan;12(1):35-45. doi: 10.1097/00003226-199301000-00007.
Human type IV collagen discs were found to support proliferation and adhesion of rabbit corneal epithelial cells in tissue culture. To assess the biocompatibility of this synthetic collagen for epikeratoplasty, seven eyes of seven rhesus monkeys underwent epikeratoplasty with lenticules made of human type IV collagen. Eye rubbing by the animals expulsed two of the lenticules and caused failure of two to epithelialize completely. The remaining three lenticules epithelialized, remained clear, and caused no adverse effects on the eye. Two of these lenticules developed focal areas of subepithelial thinning 3 months postoperatively and the third lenticule has remained stable for 30 months. The presence of epithelial attachment components at the epithelial-lenticule interface was demonstrated by immunolocalization. Histopathologic and ultrastructural examination revealed focal areas of epithelial invasion and degradation of the lenticule. Neutral proteases were detected in the thinning region of one specimen. Human type IV collagen supports epithelialization in vivo and may have potential as a biomaterial for epikeratoplasty, but the stability of the material must be improved.
在组织培养中发现人IV型胶原盘可支持兔角膜上皮细胞的增殖和黏附。为评估这种合成胶原用于表层角膜镜片术的生物相容性，对7只恒河猴的7只眼进行了用人IV型胶原制成的镜片的表层角膜镜片术。动物揉眼排出了2个镜片，并导致2个镜片完全上皮化失败。其余3个镜片上皮化，保持透明，且未对眼睛造成不良影响。其中2个镜片在术后3个月出现上皮下变薄的局部区域，第3个镜片已保持稳定30个月。通过免疫定位证明了上皮-镜片界面存在上皮附着成分。组织病理学和超微结构检查显示了镜片上皮侵袭和降解的局部区域。在1个标本的变薄区域检测到中性蛋白酶。人IV型胶原在体内支持上皮化，可能有作为表层角膜镜片术生物材料的潜力，但该材料的稳定性必须提高。
