Krüger-Krasagakes S, Li W, Richter G, Diamantstein T, Blankenstein T
Institute of Immunology, Klinikum Steglitz, Free University of Berlin, FRG.
Eur J Immunol. 1993 Apr;23(4):992-5. doi: 10.1002/eji.1830230438.
Tumor-associated eosinophils have been observed in human tumors and in experimental tumor models, but their function is poorly understood. To study the role of eosinophils during tumor growth, the plasmacytoma J558L and the mammary adenocarcinoma TS/A were transfected with an expression vector encoding the murine gene for interleukin-5 (IL-5), a cytokine inducing proliferation and activation of eosinophils. Injection of parental cells, mock-transfectants and IL-5-producing cells into syngeneic mice showed that local IL-5 secretion induced rapid tumor infiltration by eosinophils, as evidenced by immunohistochemical staining, but nevertheless did not alter the tumor growth kinetics of IL-5 transfectants. Therefore, the mere presence of IL-5 and eosinophils was not sufficient to induce a protective host immune response.
在人类肿瘤和实验性肿瘤模型中均观察到了肿瘤相关嗜酸性粒细胞，但其功能仍知之甚少。为了研究嗜酸性粒细胞在肿瘤生长过程中的作用，将浆细胞瘤J558L和乳腺腺癌TS/A用编码白细胞介素-5（IL-5）的小鼠基因的表达载体进行转染，IL-5是一种诱导嗜酸性粒细胞增殖和活化的细胞因子。将亲本细胞、mock转染细胞和产生IL-5的细胞注射到同基因小鼠体内，结果显示，免疫组化染色证明，局部IL-5分泌可诱导嗜酸性粒细胞迅速浸润肿瘤，但这并未改变IL-5转染细胞的肿瘤生长动力学。因此，仅仅存在IL-5和嗜酸性粒细胞不足以诱导宿主产生保护性免疫反应。
