Riccio M M, Manzini S, Page C P
Department of Pharmacology, King's College London, University of London, UK.
Eur J Pharmacol. 1993 Feb 23;232(1):89-97. doi: 10.1016/0014-2999(93)90732-w.
We have investigated the effects of neonatal capsaicin treatment of rabbits on the development of bronchial hyperresponsiveness following allergen exposure from birth. In vivo airways responsiveness was assessed as the concentrations of histamine to cause a 35% decrease in compliance (PC35) and a 50% increase in resistance (PC50). Rabbits treated with vehicle capsaicin (10% ethanol, 10% tween 80 and 80% saline) and then immunised with Altenaria tenius (40,000 protein nitrogen units (PNU)/ml + AL(OH)3 + saline in a ratio 2:1:1) were more responsive to aerosol histamine in vivo at three months of age when compared to sham-immunised (AL(OH)3 + saline 1:3) or saline-treated rabbits for compliance measurements (P < 0.05). However, immunised rabbits although not significantly different to sham-immunised (P > 0.05) were significantly more responsive than saline-treated rabbits for resistance measurements (P < 0.05). Neonatal capsaicin treatment significantly attenuated the increased responsiveness seen in immunised rabbits for both compliance and resistance measurements (P < 0.05). The bronchial hyperresponsiveness is unlikely to be due to cellular infiltration per se as cell numbers assessed by bronchoalveolar lavage were not significantly different between groups (P > 0.05). Exogenous in vitro functional bronchial responses to capsaicin were increased in vehicle-immunised rabbits, an effect attenuated by neonatal capsaicin treatment (P < 0.05) whereas responses to methacholine, histamine or electrical field stimulation remained similar between groups (P > 0.05). Bronchial levels of calcitonin gene-related peptide- and substance P-like immunoreactivity were unaffected by any treatment (P > 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了新生兔辣椒素处理对出生后暴露于变应原后支气管高反应性发育的影响。体内气道反应性通过引起顺应性降低35%（PC35）和阻力增加50%（PC50）的组胺浓度来评估。用赋形剂辣椒素（10%乙醇、10%吐温80和80%生理盐水）处理后，再用链格孢菌（40,000蛋白氮单位（PNU）/ml + 氢氧化铝 + 生理盐水，比例为2:1:1）免疫的兔子，与假免疫（氢氧化铝 + 生理盐水1:3）或生理盐水处理的兔子相比，在三个月大时对雾化组胺的体内反应性更高，在顺应性测量方面有统计学差异（P < 0.05）。然而，在阻力测量方面，免疫兔子虽然与假免疫兔子无显著差异（P > 0.05），但比生理盐水处理的兔子反应性显著更高（P < 0.05）。新生兔辣椒素处理显著减弱了免疫兔子在顺应性和阻力测量中出现的反应性增加（P < 0.05）。支气管高反应性不太可能是由于细胞浸润本身，因为通过支气管肺泡灌洗评估的细胞数量在各组之间无显著差异（P > 0.05）。在赋形剂免疫的兔子中，体外对辣椒素的功能性支气管反应增加，新生兔辣椒素处理可减弱这种作用（P < 0.05），而对乙酰甲胆碱、组胺或电场刺激的反应在各组之间保持相似（P > 0.05）。降钙素基因相关肽和P物质样免疫反应性的支气管水平不受任何处理的影响（P > 0.05）。（摘要截断于250字）
