Norlander T, Bolger W E, Stierna P, Uddman R, Carlsöö B
Department of Oto-Rhino-Laryngology, Karolinska Institute, Huddinge Hospital, Sweden.
Eur Arch Otorhinolaryngol. 1996;253(4-5):205-13. doi: 10.1007/BF00171129.
In order to study morphological effects on the nasal and sinus mucosa, New Zealand White rabbits underwent either unselective, regional sectioning of sensory and parasympathetic nerve branches or topical treatment of the mucosa with capsaicin. Ten days after treatment, mucosal specimens were analyzed by light and electron microscopy. Immunohistochemistry was used to evaluate neuropeptides present, in particular substance P, calcitonin gene-related peptide, vasoactive intestinal peptide and neuropeptide Y. In surgically denervated rabbits, mucosal glands were found to be enlarged and contained an increased number of zymogen granules having a bipartite substructure. Topical capsaicin application caused localized epithelial changes in the sinus mucosa and maxilloturbinal region of the nose, including clotting of cilia and an increased number of goblet cells. Reduced amounts of all neuropeptides investigated were found in the surgically denervated animals, while topical capsaicin treatment had only marginal effects on the mucosal neuropeptide content. The morphological changes observed after surgical denervation suggest an imbalance between neural stimulation and secretory capacity of the mucosal glands. These findings could explain the difference in clinical effect noted between sectioning of the vidian nerve and topical treatment with capsaicin in patients with perennial rhinitis.
为了研究对鼻和鼻窦黏膜的形态学影响，对新西兰白兔进行了感觉神经和副交感神经分支的非选择性区域切断，或用辣椒素对黏膜进行局部处理。处理后10天，通过光镜和电镜对黏膜标本进行分析。免疫组织化学用于评估存在的神经肽，特别是P物质、降钙素基因相关肽、血管活性肠肽和神经肽Y。在手术去神经支配的兔子中，发现黏膜腺增大，并且含有数量增加的具有二分亚结构的酶原颗粒。局部应用辣椒素导致鼻窦黏膜和鼻上颌鼻甲区域出现局部上皮变化，包括纤毛凝结和杯状细胞数量增加。在手术去神经支配的动物中，所有研究的神经肽含量均减少，而局部辣椒素处理对黏膜神经肽含量仅有轻微影响。手术去神经支配后观察到的形态学变化表明神经刺激与黏膜腺分泌能力之间存在失衡。这些发现可以解释在常年性鼻炎患者中，翼管神经切断术与辣椒素局部治疗之间临床效果的差异。
