Adaniya G K, Rawlins R G, Quigg J M, Roblero L, Miller I F, Zaneveld L J
Rush Medical College, Chicago, Illinois.
Fertil Steril. 1993 Mar;59(3):652-6. doi: 10.1016/s0015-0282(16)55814-2.
To determine the effect of sodium alginate encapsulation of rodent embryos on in vitro embryonic cleavage rates, implantation rates, and livebirth rates, and to find the in vivo degradation time for the capsules.
Studies were conducted using both CB6F1 mice and Golden Syrian hamsters.
Capsules made with 3.0% sodium alginate degraded in vivo within 24 to 48 hours after transfer. In vitro embryonic cleavage of encapsulated embryos was not impaired, nor were implantation rates in CB6F1 mice. Finally, 8.6% of transferred encapsulated embryos resulted in livebirths.
Encapsulation of rodent embryos in 3.0% sodium alginate is not detrimental to embryonic development, implantation rates, or fetal development. Because the capsule degrades within 48 hours after transfer, encapsulating embryos may be beneficial for human in vitro fertilization and embryo transfer.
确定海藻酸钠包裹啮齿动物胚胎对体外胚胎分裂率、着床率和活产率的影响，并找出胶囊在体内的降解时间。
使用CB6F1小鼠和金黄地鼠进行研究。
用3.0%海藻酸钠制成的胶囊在移植后24至48小时内在体内降解。包裹胚胎的体外胚胎分裂未受损害，CB6F1小鼠的着床率也未受影响。最后，8.6%的移植包裹胚胎活产。
用3.0%海藻酸钠包裹啮齿动物胚胎对胚胎发育、着床率或胎儿发育没有不利影响。由于胶囊在移植后48小时内降解，包裹胚胎可能对人类体外受精和胚胎移植有益。
