Khan-Dawood F S, Dawood M Y
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School, Houston 77030.
Fertil Steril. 1993 Apr;59(4):896-900.
To determine the effect of melatonin on baboon corpus luteum (CL) cell progesterone (P) production.
Five baboon CL obtained during the midluteal phase by luteectomy were dissociated using collagenase, and incubations were performed (50,000 cells per plate) in quadruplicate for 3 hours at 37 degrees C with melatonin (0.001 to 1.0 ng/mL) (basal) or with melatonin and 10 IU of human chorionic gonadotropin (hCG) (hCG-stimulated). Total P was measured by a specific radioimmunoassay.
Progesterone concentrations measured in the presence and absence of melatonin and hCG.
Melatonin (0.01 to 1.0 ng/mL) inhibited basal P production in all the CL (41.8 +/- 9.9 ng P without melatonin compared with 32.2 +/- 2.0 ng P, 28.4 +/- 2.1 ng with 0.01 and 1.0 ng/mL melatonin, respectively). Human chorionic gonadotropin-stimulated P production was significantly inhibited with as little as 0.01 ng of melatonin (150.8 +/- 11.4 ng with 10 IU hCG versus 120.3 +/- 6.4 ng with 10 IU hCG and 1.0 ng melatonin). The degree of inhibition in the hCG-stimulated cells was greater than in the nonstimulated cells. Melatonin at a concentration of 0.001 ng/mL did not affect P production in both stimulated and nonstimulated cells. Serotonin in similar concentrations had no effect on luteal cell P production.
These findings indicate that melatonin exerts a suppressive effect on baboon dispersed luteal cell P production and thus may play a role in luteal function.
确定褪黑素对狒狒黄体（CL）细胞孕酮（P）生成的影响。
在黄体中期通过黄体切除术获取5个狒狒黄体，用胶原酶进行解离，以每孔50,000个细胞一式四份在37℃下与褪黑素（0.001至1.0 ng/mL）（基础状态）或与褪黑素及10国际单位人绒毛膜促性腺激素（hCG）（hCG刺激状态）一起孵育3小时。通过特异性放射免疫测定法测量总P。
在有和没有褪黑素及hCG的情况下测量的孕酮浓度。
褪黑素（0.01至1.0 ng/mL）抑制了所有黄体的基础P生成（无褪黑素时为41.8±9.9 ng P，而分别添加0.01和1.0 ng/mL褪黑素时为32.2±2.0 ng P和28.4±2.1 ng P）。低至0.01 ng的褪黑素就能显著抑制hCG刺激的P生成（10国际单位hCG时为150.8±11.4 ng，而10国际单位hCG加1.0 ng褪黑素时为120.3±6.4 ng）。hCG刺激细胞中的抑制程度大于未刺激细胞。浓度为0.001 ng/mL的褪黑素对刺激和未刺激细胞中的P生成均无影响。相似浓度的血清素对黄体细胞P生成无影响。
这些发现表明褪黑素对狒狒分散的黄体细胞P生成具有抑制作用，因此可能在黄体功能中发挥作用。
