来自克氏锥虫的主要半胱氨酸蛋白酶（克氏锥虫蛋白酶）与用单克隆抗体分离出的一种抗原（Ag163B6）的鉴定。

Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody.

作者信息

Malchiodi E L, Chiaramonte M G, Martínez J A, Zwirner N W, Margni R A, Cazzulo J J

机构信息

Instituto de Estudios de la Immunidad Humoral (CONICET/UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.

出版信息

Immunol Lett. 1993 Jan;35(1):59-62. doi: 10.1016/0165-2478(93)90148-u.

DOI:10.1016/0165-2478(93)90148-u

PMID:8458637

Abstract

Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical.

摘要

通过常规方法纯化的克鲁斯蛋白酶（cruzipain）和用针对克氏锥虫提取物产生的单克隆抗体通过亲和层析分离得到的Ag163B6是具有相似电泳迁移率的糖蛋白，它们与大多数慢性恰加斯病患者的血清发生反应。它们在SDS-PAGE、蛋白质免疫印迹、等电聚焦、二维电泳（IEF和SDS-PAGE）、双向免疫扩散以及在含有0.1%明胶的SDS-PAGE凝胶中的酶活性方面的表现表明它们是相同的。