大肠杆菌中KatF（σS）的合成受转录后调控。
KatF (sigma S) synthesis in Escherichia coli is subject to posttranscriptional regulation.
作者信息
Loewen P C, von Ossowski I, Switala J, Mulvey M R
机构信息
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
出版信息
J Bacteriol. 1993 Apr;175(7):2150-3. doi: 10.1128/jb.175.7.2150-2153.1993.
Abstract
A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.
摘要
katF与lacZ基因的转录融合在整个指数生长期表达水平逐渐升高，但翻译融合在指数生长期生长时表达水平较低，而在向稳定期转变过程中被诱导160倍，这表明KatF合成的调控存在转录后机制。突变分析表明，katF的起始密码子是先前鉴定的开放阅读框中的第二个ATG。
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